Coding

Part:BBa_K5102003

Designed by: Lucas Mair   Group: iGEM24_Munich   (2024-09-29)


mTagBFP2

The mTagBFP2 part encodes a blue fluorescent protein characterized by an excitation wavelength of 399 nm and an emission wavelength of 454 nm. It has been specifically codon-optimized for expression in mammalian cells. Importantly, the design guarantees that there are no STOP codons present in any of the three forward open reading frames, allowing for flexible readout design tailored to ones project. This adaptability enables researchers to utilize the part in various applications.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 511
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 602
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None